diff --git a/Lib/test/test_support.py b/Lib/test/test_support.py
index 97db7405a8bbef..d786aac3aede9f 100644
--- a/Lib/test/test_support.py
+++ b/Lib/test/test_support.py
@@ -567,7 +567,6 @@ def test_args_from_interpreter_flags(self):
             ['-X', 'int_max_str_digits=1000'],
             ['-X', 'lazy_imports=all'],
             ['-X', 'no_debug_ranges'],
-            ['-X', 'pycache_prefix=/tmp/pycache'],
             ['-X', 'showrefcount'],
             ['-X', 'tracemalloc'],
             ['-X', 'tracemalloc=3'],
@@ -576,6 +575,12 @@ def test_args_from_interpreter_flags(self):
             with self.subTest(opts=opts):
                 self.check_options(opts, 'args_from_interpreter_flags')
 
+        with os_helper.temp_dir() as temp_path:
+            prefix = os.path.join(temp_path, 'pycache')
+            opts = ['-X', f'pycache_prefix={prefix}']
+            with self.subTest(opts=opts):
+                self.check_options(opts, 'args_from_interpreter_flags')
+
         self.check_options(['-I', '-E', '-s', '-P'],
                            'args_from_interpreter_flags',
                            ['-I'])